3,3',5,5'-Tetramethylbenzidine（TMB）(Biological level)
Appearance:white crystal powder
Purity:≥99% ;Molecular formula:C16H20N2 ;Molecular wt:240.35
Melting point:169-170οC ;CAS No:54827-17-7 ;Prod No.:TC-001 ;
Producing Area:Luoyang China
The performance and application of TMB:
3,3',5,5'-Tetramethylbenzidine(TMB) is a new-type safe color primary reagent.Compared with some traditional color primary reagents such as benzidine, o-tolidine and o-diaminobenzene,the merits of TMB are high sensitive detection and excellent stability. And as a result of zoopery and Ames which is to experiment on self-acting variability of bacteria,showing that TMB is a kind of non-cancerogen and non-mutagen ,and can be used safely. Currently,TMB is taking the place of benzidine which is a strong cancerogen and other ramifications of benzidine which are carcinogenic step by step.TMB can be applied in areas like clinical assay, forensic analysis, criminal investigation and environmental detection,etc.As a new zymolyte of peroxidase,TMB has wide applications in enzyme immunoassay (EIA) and enzyme-linked-immunosorbent serologic assay (ELISA).
The main items of TMBs applications are:
1. making up test paper of uranalysis;
2. detection of hepatitis virus;
3. detection of gravidity;
4. rapid determination of glucose,hemoglobin and albumin in blood and urine;
5. occult blood test of excrement;
6. to detect the value of granulocyte in blood;
7. detection of steroid and sex hormone;
8. determination of enzyme activity;
9. analysis and detection of antigen, antibody and germ plasm;
10. rapid determination of alcoholicity in saliva;
11. pigmentation of biological specimen;
12. quality test of water and detection of fingerprint rendering;
13. detection of AIDS virus.
Properties of TMB:
White / primrose yellow crystalline powder, odourlessness and tastelessness. Difficult to dissolve in the water, but easy to dissolve in the organic solvents such as acetone, aether, dimethylsulphoxide and dimethylformamide, etc. |
